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Pertussis toxin-induced inhibition of Wnt/?-catenin signaling in dendritic cells promotes an autoimmune response in experimental autoimmune uveitis

Zhang, Z;Li, Y;Chen, N;Li, H;Chen, S;Cui, X;Shao, H;Wei, L;Ma, J;Zhang, S;Li, X;Zhang, X;

Previous reports have indicated that disrupting the Wnt/?-catenin pathway in dendritic cells (DCs) may affect the progression of autoimmune inflammation; however, the factors and timing that regulate Wnt/?-catenin signaling have not been clearly understood.Experimental autoimmune uveitis (EAU) mice and Vogt-Koyanagi-Harada disease (VKH) patient samples were used to detect the expression of Wnt/?-catenin pathway genes. Western blot, real-time PCR, flow cytometry, and ELISA were performed to examine the expression of components of the Wnt/?-catenin pathway and inflammatory factors. DC-specific ?-catenin knockout mice and 6-bromoindirubin-3'-oxime (BIO) administered mice were used to observe the effect of disrupting the Wnt pathway on EAU pathogenesis.Wnt/?-catenin signaling was inhibited in DCs during the induction phase of EAU. The inhibition was mediated by pertussis toxin (PTX), which promoted DC maturation, in turn promoting pathogenic T cell proliferation and differentiation. In vivo experiments confirmed that deleting ?-catenin in DCs enhanced EAU severity, and pre-injection of PTX advanced EAU onset. Administration of a Wnt activator (BIO) limited the effects of PTX, in turn ameliorating EAU.Our results demonstrate that PTX plays a key role as a virulence factor in initiating autoimmune inflammation via DCs by inhibiting Wnt/?-catenin signaling in EAU, and highlight the potential mechanism by which infection can trigger apparent autoimmunity.